Generator

Part:BBa_K1520008

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-22)

Pcons2-rbs-lacI-Ter

Pcons2-rbs-lacI-Ter. It constantly produces lacI.

K152008.jpg

From left to right, Marker (DL10000), Pcons2, Pcons2-lacI, Pcons-lacI-Ter.

Primers used in PCR are VF2 and VR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
directionForward
functionRep
ligandsallolactose, IPTG
n/aconstitutive promoter family member
negative_regulators
positive_regulators
proteinLacI
swissproP03023
tagLVA